How can i collect 24hours urine for respectively one of 60 rats ?

i want to collect 24 hours urine for 60 rats separately to investigate for urea clearance rate and creatinine clearance rate


Answers:    I have done this, years ago. character above had it right so far as the seperation of cage and the wire floors... But you want larger mesh, to some extent than smaller. Comfortable for the rat, and small enough to block feces.

stretch saran wrap over the bottom of each pen with a can jar ring in the middle of respectively floor facing down. Be a little unstinting with the wrap, but keep hold of it taut. You may want to tie it on beside wire to be sure you do not lose one. I didn't, and i lost one.

Grease the border of a jar and screw it on to the lid through the wrap. For rats small jars work best (otherwise you own these great big jars beside a dribble in the bottom of each) If you live surrounded by a decent sized city you can seize pretty small ones intended for gifts of jam.

Shim the jar so that a small amount of tension is on the wrap, and the urine will be flowing toward the ring lid.

Poke a small hole contained by the wrap with a blown out wooden clash.

the urine of each rat will run down the wrap, through the hole, and collect surrounded by his jar, there will be little evaporation.

If you obligation the urine fresh, you will need to wrap the cup jars contained by ice.

If bacterial accomplishment must be avoided you may need rime and you will have to hold on to it all severely clean.

If what you are trialling for is degraded by light, you will enjoy to put blackout covers on the jars, or paint them black... surrounded by that case do not forget to cut a black-out ring for most of the top of the jar as powerfully.

If whatever you are doing near the urine will not be ruined by a stablizer or neutralizer, and you plan to use one, you can put it in the jar ahead of time.

I have not tested for those things specificly, but i do deem there is chagrin with temp and germs of those two, but that light will not issue.
Get a large coop with a fine mesh bottom so the little friends can tramp. Put slats in between respectively furry friend that separate both the upper compartments (to keep respectively rat and his/her urine isolated). When you are done constructing this (use all metal so no urine is lost through incorporation into wood) the complex should look like a 30-day pill-minder container approaching they sell at the drugstore. Use hulking rats of similar weight so they adjectives produce a greater amount of urine so it won't all be lost due to evaporation. Good luck on your project. FYI--Texas catheter on masculine dog would be a lot easier, LOL
NurseMom

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