While in the lab, How is the CBC with differential done? What kind of machine is it done on?
Answer:
CBC is drawn is a purple tube which contains the anticoagulant EDTA. Tube must be mixed (inverted) to prevent clotting of the blood. Labs perform a CBC on a Hematology analyzer -- many are on the market by a number of different companies (Coulter, Sysmex - to name two). The results - RBC, WCB, PLT count, Hct, Hgb and RBC indices as well as the automated differential count are evaluated by a Medical Technologist (MT or MLT) and based on the results they will decided if the automated differential is acceptable or if a manual diff. is needed. For the manual diff a blood smear is made, dried, and stained. The tech will then view the slide on the microscope counting and differentiating 100 cells. Additionally, the tech will evaluate plt count and RBC morphology -- size and shape.
CBC with differential are draw in a lavender tube the addditive is Ethylenediaminetetraacetic acid also known as EDTA differential diagnosis is diagnosis based on comparison of symptoms of similar diseases
Agree with Kassey. In addition, the instrumentation that performs the testing, passes the cellular material from the EDTA tube through several small appertures that measure many aspects of the cells such as volume (size of the cell), conductivity (the amount of electrical current that passes through the cell), and light scatter (how much light bounces off the cell through the use of a laser). In addition, some newer technology also tags the cellular material with chemical fluorescent markers that cause the cells to glow at varying levels of intensity. These are also measured. Once all the data is gathered by the instrument, it uses complex mathematical formulas and algorythms to determine the numerical results of the CBC. The amazing part about this process is that it all occurs in under 60 seconds and the instrument moves on to the next sample to begin the process again.
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