PCR with two primers, one negative control.?
I assume insulin is the negative control. There is an insulin band in all the columns but what is it for?
Answer:
I would call it an internal control, not a negative control. The intensity of the insulin band should be the same in all of the lanes. This tells you that you started the PCR with the same amount of template DNA in each tube and that the same reaction occurred in each tube.
If the intensity of the insulin band varies, then that means your tubes either started with different amounts of template DNA, or there was something else different about the reaction mixture. Whatever the case, you won't be able to derive quantitative conclusions from the ovalbumin band intensities unless your insulin bands are all the same.
Unless, of course, you weren't supposed to see insulin bands at all. You did not say what experiment you were actually doing. If your ovalbumin gene is in a bacterial plasmid for example, and there isn't supposed to be an insulin gene there, then having an insulin band means that you contaminated your reaction tubes--probably with your own DNA. In this case, the insulin band *is* supposed to be a negative control (you aren't supposed to see it).
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