Why is the germs steam fixed to slide using the bunsen burner??
Answers:
It so that the bacteria isn't dust away during the staining process. It also kills the microbes to prevent them from being a hazard to the stainer. The heat fixing technique is used contained by most staining techniques ie TB staining, it however isn't used surrounded by the staining of cryptosporidium as it would kill the parasite. You also want to bring in sure that your smear of the organsim on the slide isn't to thick as it will be wash away even after heat fixing, one to thick can also affect the staining process and prevent the stains from man absorbed properly.
The gram stain differentiates microbes into 2 easily recognisable group Gram +(purple) and Gram -(pink). The differences between these 2 groups of organism is the compactness of their peptidoglycan layer which is located surrounded by the cell wall.
Gram + organisms have thicker pepetidoglycan layer so they retain the first stain which is crystal violet, iodine is added which act as a mordant, these 2 stains form the crystal violet-iodine complex that is not slickly removed by the addition of alcohol/acetone. The counter stain carbol fuscin/neural red is added and can't be obsessed due to the already formed complex. It is simply washed away. Therefore the organisms appear purple below the microscope.
Gram - organism have thinner peptidoglycan band so the first stain Crystal violet is wash away. So there is no formation of a crystal violet-iodine complex so the finishing stain Carbol fuscin/neutral red is the one that is enthralled. Therfore the organism appear pink under the microscope.
The gram stain be developed by Hans Christian Gram in 1882.
Its resembling when you burn something you're cooking; notice how it sticks to the vessel and won't come off no business what you do? Same idea beside heat fixing, but on a much smaller amount. Then when you do the multiple washes involved contained by any staining process (gram stain, acid swiftly stain, etc) the bacteria will stay put on the slide fairly than washing into the sink.
Fixing it to the slide is one purpose to fry fixing. It also kills most germs, so you lessen the odds of infecting yourself or contaminating your work nouns.
It's done for every type of staining I've done, not just gram stains
This is simple process to allow the microbes to adhere to the slide instead of self rinsed off by the force of the stain and rinse of the gram stain process. Even next to this some specimen can be removed if care is not exercised within the process.
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